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pdonor tbfp nls neo universal donor  (Addgene inc)


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    Structured Review

    Addgene inc pdonor tbfp nls neo universal donor
    Pdonor Tbfp Nls Neo Universal Donor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdonor+tbfp+nls+neo+universal+donor/pDonor-tBFP-NLS-Neo+(Universal)+(Plasmid+%2380767)/pm35582972-134-23-28
    Average 93 stars, based on 21 article reviews
    pdonor tbfp nls neo universal donor - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: DYRK2 maintains genome stability via neddylation of cullins in response to DNA damage.
    Article Snippet: Briefly, two independent single-guide RNA (sgRNA) sequences targeting the human DYRK2 gene (#1: 5′-CCTGGATCTGTCCGTGAGCG-3′) and (#2: 5′-GAGCCCGGTAAAAACGCGAC-3′) were designed and inserted into peSpCAS9(1.1)-2×sgRNA (Addgene plasmid #80768). .. The hTERT-RPE1 cells were transfected Jo u rn al o f Ce ll Sc ie n ce with the sgRNA vector and the pDonor-tBFP-NLS-Neo (Universal) donor knock-in vector (Addgene plasmid #80767) via a X-tremeGENETM9 transfection reagent (Roche Applied Science), selected by 600 μg/ml G418, and subcloned. ..

    Article Title: Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking
    Article Snippet: .. Double-stranded oligonucleotides for the target sequences were inserted into peSpCAS9(1.1)-2 × sgRNA (Addgene 80768), an all-in-one sgRNA expression vector. hTERT-RPE1 cells grown on a 12-well plate were transfected with the sgRNA vector and the pDonor-tBFP-NLS-Neo(universal) donor knock-in vector (Addgene 80767) using X-tremeGENE9 transfection reagent (Roche Applied Science). ..

    Article Title: Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking
    Article Snippet: .. Double-stranded oligonucleotides for the target sequences were inserted into peSpCAS9(1.1)-2×sgRNA (Addgene 80768), an all-in-one sgRNA expression vector. hTERT-RPE1 cells grown on a 12-well plate were transfected with the sgRNA vector and the pDonor-tBFP-NLS-Neo(universal) donor knock-in vector (Addgene 80767) using X-tremeGENE9 transfection reagent (Roche Applied Science). ..

    Knock-In:

    Article Title: DYRK2 maintains genome stability via neddylation of cullins in response to DNA damage.
    Article Snippet: Briefly, two independent single-guide RNA (sgRNA) sequences targeting the human DYRK2 gene (#1: 5′-CCTGGATCTGTCCGTGAGCG-3′) and (#2: 5′-GAGCCCGGTAAAAACGCGAC-3′) were designed and inserted into peSpCAS9(1.1)-2×sgRNA (Addgene plasmid #80768). .. The hTERT-RPE1 cells were transfected Jo u rn al o f Ce ll Sc ie n ce with the sgRNA vector and the pDonor-tBFP-NLS-Neo (Universal) donor knock-in vector (Addgene plasmid #80767) via a X-tremeGENETM9 transfection reagent (Roche Applied Science), selected by 600 μg/ml G418, and subcloned. ..

    Article Title: Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking
    Article Snippet: .. Double-stranded oligonucleotides for the target sequences were inserted into peSpCAS9(1.1)-2 × sgRNA (Addgene 80768), an all-in-one sgRNA expression vector. hTERT-RPE1 cells grown on a 12-well plate were transfected with the sgRNA vector and the pDonor-tBFP-NLS-Neo(universal) donor knock-in vector (Addgene 80767) using X-tremeGENE9 transfection reagent (Roche Applied Science). ..

    Article Title: Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking
    Article Snippet: .. Double-stranded oligonucleotides for the target sequences were inserted into peSpCAS9(1.1)-2×sgRNA (Addgene 80768), an all-in-one sgRNA expression vector. hTERT-RPE1 cells grown on a 12-well plate were transfected with the sgRNA vector and the pDonor-tBFP-NLS-Neo(universal) donor knock-in vector (Addgene 80767) using X-tremeGENE9 transfection reagent (Roche Applied Science). ..

    Plasmid Preparation:

    Article Title: DYRK2 maintains genome stability via neddylation of cullins in response to DNA damage.
    Article Snippet: Briefly, two independent single-guide RNA (sgRNA) sequences targeting the human DYRK2 gene (#1: 5′-CCTGGATCTGTCCGTGAGCG-3′) and (#2: 5′-GAGCCCGGTAAAAACGCGAC-3′) were designed and inserted into peSpCAS9(1.1)-2×sgRNA (Addgene plasmid #80768). .. The hTERT-RPE1 cells were transfected Jo u rn al o f Ce ll Sc ie n ce with the sgRNA vector and the pDonor-tBFP-NLS-Neo (Universal) donor knock-in vector (Addgene plasmid #80767) via a X-tremeGENETM9 transfection reagent (Roche Applied Science), selected by 600 μg/ml G418, and subcloned. ..

    Article Title: Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking
    Article Snippet: .. Double-stranded oligonucleotides for the target sequences were inserted into peSpCAS9(1.1)-2 × sgRNA (Addgene 80768), an all-in-one sgRNA expression vector. hTERT-RPE1 cells grown on a 12-well plate were transfected with the sgRNA vector and the pDonor-tBFP-NLS-Neo(universal) donor knock-in vector (Addgene 80767) using X-tremeGENE9 transfection reagent (Roche Applied Science). ..

    Article Title: Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking
    Article Snippet: .. Double-stranded oligonucleotides for the target sequences were inserted into peSpCAS9(1.1)-2×sgRNA (Addgene 80768), an all-in-one sgRNA expression vector. hTERT-RPE1 cells grown on a 12-well plate were transfected with the sgRNA vector and the pDonor-tBFP-NLS-Neo(universal) donor knock-in vector (Addgene 80767) using X-tremeGENE9 transfection reagent (Roche Applied Science). ..

    Expressing:

    Article Title: Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking
    Article Snippet: .. Double-stranded oligonucleotides for the target sequences were inserted into peSpCAS9(1.1)-2 × sgRNA (Addgene 80768), an all-in-one sgRNA expression vector. hTERT-RPE1 cells grown on a 12-well plate were transfected with the sgRNA vector and the pDonor-tBFP-NLS-Neo(universal) donor knock-in vector (Addgene 80767) using X-tremeGENE9 transfection reagent (Roche Applied Science). ..

    Article Title: Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking
    Article Snippet: .. Double-stranded oligonucleotides for the target sequences were inserted into peSpCAS9(1.1)-2×sgRNA (Addgene 80768), an all-in-one sgRNA expression vector. hTERT-RPE1 cells grown on a 12-well plate were transfected with the sgRNA vector and the pDonor-tBFP-NLS-Neo(universal) donor knock-in vector (Addgene 80767) using X-tremeGENE9 transfection reagent (Roche Applied Science). ..



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